Department 	Biology 	
Module Name: 	Plant Tissue Culture and Food Biotechnology	
Course Title: 	Introduction to Plant Tissue Culture and Propagation	
Course Code: 	Biol 3131	
Course Credit	2 (1 theoretical classes + 1 practical /week)	
Course Credit point: 	3ECTS	
 Pre-requisite	Plant Physiology (Biol 2024)	
Academic Year	2020	
Semester 	II	
Target group	III year ,II semester Biology major students	
Course status	Elective	
Instructors contact information	
Instructors Name	Belachew, B	
E-mail	Belachew2145@gmail.com	
Office Number		
Office phone number 	+251919130793	
Office hour		
Class schedule 		
Venue; 		
     Course Description:
Laboratory organization of plant tissue culture; medium and sterilization techniques; different types of cultures; micro propagation; cell suspension and secondary metabolites; in vitro production of haploids; protoplast isolation and fusion; somaclonal variation; germ plasm storage and cryopreservation; micro propagation and other propagation methods
  
Course objectives  
After completing the course, the students should be able to:
	Master the basic principles and skills regarding techniques, practices and procedures of plant tissue culture (Micro propagation), asepsis, laboratory plan, equipment and facilities, and green house growing.
	Discuss applications of plant tissue culture.
	Practice the most common methods of plant cell and tissue culture. 
	Propagate rare and endangered plant species by laboratory method.
Time	Topics/Contents	Readings 	
Week one 	1. Introduction 
1.1. Definition
1.2. Applications
1.3. History
	
George, E., M. Hall, G. Klerk (eds.), 2007. Plant propagation by tissue culture. 3
 edition.vol.1 
	
Week  two 	2. Facilities and supplies 
2.1. Organization of a tissue culture laboratory
2.2. Equipment and supplies
2.3. Basic technique
	George, E., M. Hall, G. Klerk (eds.), 2007. Plant propagation by tissue culture. 3
 edition.vol.1 

Week three 	3. Tissue culture media   
3.1. Media components
3.2. Media preparation
4. Callus and cell culture 
4.1. Callus induction
4.2. Callus culture
4.3. Cell suspension culture	 
George, E., M. Hall, G. Klerk (eds.), 2007. Plant propagation by tissue culture. 3
 edition.vol.1 	
Week Four 	5. Micro propagation 
5.1. Applications
5.2. Micro propagation types
5.3. Micro propagation stages	George, E., M. Hall, G. Klerk (eds.), 2007. Plant propagation by tissue culture. 3
 edition.vol.1 

Week Five 	6. Organogenesis & Embryogenesis 
  Anther/microspore culture& 
Embryo culture 
	
George, E., M. Hall, G. Klerk (eds.), 2007. Plant propagation by tissue culture. 3
 edition.vol.1 	
Week six 	
7. Somaclonal variation & Plant transformation 
	
George, E., M. Hall, G. Klerk (eds.), 2007. Plant propagation by tissue culture. 3
 edition.vol.1 	
Week Seven 	8. Germ plasm preservation 	 
George, E., M. Hall, G. Klerk (eds.), 2007. Plant propagation by tissue culture. 3
 edition.vol.1 
	
Mode of Assessment Methods:
Mid exam ……………………………………………....30%
Laboratory Activities and Reports……………………... 20%
Final written exam………………….……..…………… 50%
Total ……………………………………………………100%
8. References: 
1.	Agrawal, R.L., 1998.Fundamentals of plant breeding and hybrid seed production. Oxford and IBH publishing, New Delhi.
2.	Bhojwani, S. and M. Razdan, 1996. Plant tissue culture: Theory and Practice. Department of Botany University of Delhi. Amsterdams, the Netherlands, revised edition. pp. 26 - 33
3.	George, E., M. Hall, G. Klerk (eds.), 2007. Plant propagation by tissue culture. 3edition.vol.1 
4.	IAEA (International Atomic Energy Agency), 2004. Low cost options for tissue culture technology in developing countries.  Proceedings of a technical meeting organized by the joint FAO/IAEA division of nuclear techniques in food and agriculture and held in Vienna, 26–30. http://www-pub.iaea.org/MTCD/publications/PDF/te_1384_web.pdf
5.	Mineo, L., 1990. Plant tissue culture techniques.http://www.ableweb.org/volumes/vol11/9-mineo.pdf
6.	PTL (PhytoTechnology Laboratories), 2008. African violet multiplication kit. http://www. phytotechlab.com/ pdf/ African VioletKit.pdf
7.	Seymour, W. Mitchell and M. Ahmad, 2003. A novel surface sterilization method for reducing microbial contamination of field grown medicinal explants intended for in vitro culture. Biotechnology Centre, U.W.I, Mona, Kingston 7, Ja. W.I.Part II